Background
Common carp is the 3rd most widely cultivated fresh water fish species in the world (FAO, 2018). It is a widespread fresh water fish of eutrophic waters in lakes and large rivers of Europe and Asia, but present in brackish water also. The native wild populations are considered vulnerable to extinction by the International Union for Conservation of Nature (IUCN), but the species has also been domesticated and introduced into environments worldwide. It is having both monoculture and polyculture practices.
Key Activities
To assemble stocks of common carp from different geographical areas
To evaluate the performance of common carp in various salinities
To estimate the genetic parameters of economic traits
Assembling & Rearing of Common Carp
Assembling of Stocks
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Andhra Pradesh Stock:
The broodstocks of common carp was procured from various locations in Andhra Pradesh.
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Orissa Stock:
The brooders were procured from National Freshwater Fish Broodbank, Bhubaneshwar
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Tamil Nadu Stock:
The brooders were procured from fish tanks nearby Chennai.
Designing and Production of Families
A total of 78 families were produced viz., 20 full sib families Madhya Pradesh stock, 24 full sib families -Andhra Pradesh stock, 29 families viz., 22 full sib and 7 half sib families belonging to Haryana stock, 5 full-sib families- Tamil stock, were produced and reared at ICAR-CIFE, Rohtak center separately in rearing hapas till they attain taggable size. A total of 2200 fish belonging to Sixty-two families (s) were PIT tagged and released in two grow-out ponds of two different salinities (1. Four ppt salinity and 2. Eight ppt salinity). The traits viz, Bodyweight, Standard length and Body depth of tagged fish was recorded prior to release.
Acclimatization and Water Quality Analysis
Acclimatization and Rearing
Regular sampling and recording of performance traits at two salinities was done. The sampling was done four times during the reporting period which is 200 days’ pond culture age. The between and within family variation for performance traits in common carp was observed. The estimation of genetic parameters for growth traits at three months of pond age is in progress.
For genetic characterisation of stocks the DNA was extracted from 300 fin tissue samples and quality check was conducted. The primers for microsatellite loci and D loop were standardised using gradient PCR. The genotying for microsatellite loci and sequencing for D loop is in progress.
Water quality parameters
All the water quality parameters were checked after stocking the Common carp families in the partitioned pond.
Recording of temperature and DO of pond water is being carried out every day after stocking.